Map2k3^tm1Flv
Targeted Allele Detail

Summary

• Symbol: Map2k3^tm1Flv
• Name: mitogen-activated protein kinase kinase 3; targeted mutation 1, Richard A Flavell
• MGI ID: MGI:1857752
• Synonyms: Mkk3-, MKK3 KO
• Gene: Map2k3 Location: Chr11:60822880-60843637 bp, + strand Genetic Position: Chr11, 37.95 cM
• Alliance: Map2k3^tm1Flv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54309
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a 1.5kb region that includes exons 8 and 9, which encode amino acids 217-221 of the protein. This region includes the sequences containing the dual phosphorylation sites (serine and threonine) that are required for activation of the protein. Northern blot and Western blot analyses did not detect expression from this allele in homozygous mutant mice. (J:54309)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Map2k3 Mutation: 30 strains or lines available

Notes

Homozygous mutant mice are viable and appear normal, healthy, and fertile with no defects in lymphocyte development (thymocytes, splenocytes, and bone-marrow derived dendritic cells). Compared to control sibs, however, macrophages and primary embryonic fibroblasts from mutant mice show reduced activation of p38 MAPK. Cytokine IL-12 (encoded for by Il12a and Il12b) is strongly reduced in mutant macrophages and dendritic cells. Mutant fibroblasts have a defective response to Tnf. Mutant mice also have defective T-helper cells with respect to the ability to produce Ifng in response to antigen stimulation of keyhole limpet hemocyanin (KLH) both in vitro and in vivo (J:54078, J:54309).

References

• Original: J:54309 Lu HT, et al., Defective IL-12 production in mitogen-activated protein (MAP) kinase kinase 3 (Mkk3)-deficient mice. EMBO J. 1999 Apr 1;18(7):1845-57
• All: 47 reference(s)