Parp1^tm1Hsmm
Targeted Allele Detail

Summary

• Symbol: Parp1^tm1Hsmm
• Name: poly (ADP-ribose) polymerase family, member 1; targeted mutation 1, Hiroshi Suzuki Mitsuko Masutani
• MGI ID: MGI:1857765
• Synonyms: Parp^-
• Gene: Parp1 Location: Chr1:180396456-180428564 bp, + strand Genetic Position: Chr1, 84.44 cM, cytoband H5
• Alliance: Parp1^tm1Hsmm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53360
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into exon 1, 10 bp downstream of the ATG codon. Protein activity was not detected in tissues derived from homozygous mice. Western blot analysis detected a 113 kDa protein in homozygous mice, but this truncated form is predicted to be inactive beacuse it should lack a critical N-terminal zinc finger. (J:53360)

Disease models

Tumor Data

List all tumor models in MMHCdb carrying Parp1^tm1Hsmm

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 8 strains available Cell Lines: 0 lines available
• Carrying any Parp1 Mutation: 66 strains or lines available

Notes

Null mice appear healthy and fertile with no gross abnormalities. No skin hyperplasia or obesity was observed. These mice were used to examine the role of ADPRP in Streptozotocin induced diabetes. STZ injection caused a significant increase in blood glucose concentrations of wildtype mice as well as necrosis in islet cells leading to an eventual loss on beta cells. In contrast, homozygous mice are protected from STZ-induced diabetes. Glucose levels in these animals remained relatively normal and islet beta cell death was markedly less in these animals (J:53360).

References

• Original: J:53360 Masutani M, et al., Poly(ADP-ribose) polymerase gene disruption conferred mice resistant to streptozotocin-induced diabetes. Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2301-4
• All: 15 reference(s)