Gli1^tm1Alj
Targeted Allele Detail

Summary

• Symbol: Gli1^tm1Alj
• Name: GLI-Kruppel family member GLI1; targeted mutation 1, Alexandra L Joyner
• MGI ID: MGI:1857797
• Synonyms: Gli1^-, Gli1^zfd
• Gene: Gli1 Location: Chr10:127165751-127177448 bp, - strand Genetic Position: Chr10, 74.5 cM
• Alliance: Gli1^tm1Alj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60986
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced exons 9 to 11, which encode part of zinc finger 1, zinc fingers 2 - 5, and 43 adjacent amino acids. Northern blots of total RNA from E13.5 embryos using a cDNA probe 3' to the zinc fingers resulted in detection of 2 transcripts in wild-type embryos and only one smaller broad band in homozygous mutant embryos. Further analysis by RT-PCR followed by sequencing of the products showed that the mutant allele produced a transcript that spliced around the neomycin insert from exon 8 - 12. The authors state that as a result, the mutant allele could not encode for a protein with DNA-binding activity, but could produce a mutant protein lacking amino acids 269 - 437. (J:60986)

Expression

In Mice Carrying this Mutation:

121 assay results

Tumor Data

List all tumor models in MMHCdb carrying Gli1^tm1Alj

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gli1 Mutation: 49 strains or lines available

References

• Original: J:60986 Park HL, et al., Mouse Gli1 mutants are viable but have defects in SHH signaling in combination with a Gli2 mutation. Development. 2000 Apr;127(8):1593-605
• All: 28 reference(s)