Aqp1^tm1Ask
Targeted Allele Detail

Summary

• Symbol: Aqp1^tm1Ask
• Name: aquaporin 1; targeted mutation 1, Alan S Verkman
• MGI ID: MGI:1857807
• Synonyms: AQP1-
• Gene: Aqp1 Location: Chr6:55313417-55325540 bp, + strand Genetic Position: Chr6, 27.38 cM
• Alliance: Aqp1^tm1Ask page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45888
• Parent Cell Line: CB1-4 (ES Cell)
• Strain of Origin: C57BL/6J x (Rb(11.16)2H x Rb(16.17)32Lub)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 1.8 kb neomycin cassette replaced part of the exon 1 coding sequence and ~1.3 kb of intron 1. Northern blot analysis of kidney, lung, eye, spleen, and heart homogenates from homozygous mutant mice did not detect full-length transcripts. Western blots of kidney, lung, eye, spleen, and heart homogenates from homozygous mutant mice did not detect showed no detectable encoded protein. (J:45888)

Expression

In Mice Carrying this Mutation:	224 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aqp1 Mutation: 23 strains or lines available

References

• Original: J:45888 Ma T, et al., Severely impaired urinary concentrating ability in transgenic mice lacking aquaporin-1 water channels. J Biol Chem. 1998 Feb 20;273(8):4296-9
• All: 85 reference(s)