Aqp4^tm1Ask
Targeted Allele Detail

Summary

• Symbol: Aqp4^tm1Ask
• Name: aquaporin 4; targeted mutation 1, Alan S Verkman
• MGI ID: MGI:1857808
• Synonyms: AQP4-
• Gene: Aqp4 Location: Chr18:15522553-15544039 bp, - strand Genetic Position: Chr18, 8.74 cM
• Alliance: Aqp4^tm1Ask page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:42704
• Parent Cell Line: CB1-4 (ES Cell)
• Strain of Origin: C57BL/6J x (Rb(11.16)2H x Rb(16.17)32Lub)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced part of the exon 1 coding sequence (bp 298 - 381) into ~1kb of intron 1. Northern blots of brain homogenates from homozygous mutant mice showed minimally detectable truncated Aqp4 transcripts. Western blots of brain homogenates from homozygous mutant mice showed no detectable Aqp4 protein. Immunocytochemistry of renal tissue from homozygous mutant mice showed no detectable Aqp4 protein. (J:42704)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Aqp4 Mutation: 46 strains or lines available

References

• Original: J:42704 Ma T, et al., Generation and phenotype of a transgenic knockout mouse lacking the mercurial-insensitive water channel aquaporin-4. J Clin Invest. 1997 Sep 1;100(5):957-62
• All: 73 reference(s)