Scp2^tm1Usee
Targeted Allele Detail

Summary

• Symbol: Scp2^tm1Usee
• Name: sterol carrier protein 2, liver; targeted mutation 1, Udo Seedorf
• MGI ID: MGI:1857809
• Synonyms: Scp2 -
• Gene: Scp2 Location: Chr4:107901027-108002168 bp, - strand Genetic Position: Chr4, 50.2 cM
• Alliance: Scp2^tm1Usee page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:47280
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 14 was disrupted by the insertion of a neomycin cassette. Sequence analysis of truncated transcript identified by Northern analysis, showed that an aberrant splice pattern resulted in the excision of exon 14. The joining of exons 13 and 15 resulted in a frameshift mutation which introduced a stop codon in exon 15, upstream of an essential peroxisomal targeting signal. Western analysis revealed an absence of normal protein in the livers of homozygous mutant mice. (J:47280)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Scp2 Mutation: 59 strains or lines available

References

• Original: J:47280 Seedorf U, et al., Defective peroxisomal catabolism of branched fatty acyl coenzyme A in mice lacking the sterol carrier protein-2/sterol carrier protein-x gene function. Genes Dev. 1998 Apr 15;12(8):1189-201
• All: 5 reference(s)