Pitx2^tm2Sac
Targeted Allele Detail

Summary

• Symbol: Pitx2^tm2Sac
• Name: paired-like homeodomain transcription factor 2; targeted mutation 2, Sally A Camper
• MGI ID: MGI:1857846
• Synonyms: Pitx2^-, Pitx2^null
• Gene: Pitx2 Location: Chr3:128993527-129013240 bp, + strand Genetic Position: Chr3, 57.84 cM
• Alliance: Pitx2^tm2Sac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:51160
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele is derived from Pitx2^tm1Sac. Gene targeting was performed with a vector engineered to contain a loxP site downstream from exon 4 and a floxed neomycin cassette upstream of exon 4. Subsequent cre expression resulted in deletion of both the neo selection cassette and exon 4. This allele is a demonstrated to be a functional null. (J:51160)

Disease models

Expression

In Mice Carrying this Mutation:	172 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	23 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pitx2 Mutation: 39 strains or lines available

References

• Original: J:51160 Gage PJ, et al., Dosage requirement of Pitx2 for development of multiple organs. Development. 1999 Oct;126(20):4643-51
• All: 35 reference(s)