Hnf1b^tm1Sce
Targeted Allele Detail

Summary

• Symbol: Hnf1b^tm1Sce
• Name: HNF1 homeobox B; targeted mutation 1, Silvia Cereghini
• MGI ID: MGI:1857856
• Synonyms: Hnf1b^lacZ, vHnf1^-, vHNF1^{deltaExon1-SC}
• Gene: Hnf1b Location: Chr11:83741035-83796743 bp, + strand Genetic Position: Chr11, 51.23 cM
• Alliance: Hnf1b^tm1Sce page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58079
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 was replaced by a construct containing lacZ and neo. The deleted coding sequence encodes the entire N-terminal dimerization domain and a portion of another domain showing homology to either the human POU-A subdomain or human B-domain. Western blot analysis showed an absence of encoded protein in homozygous mutant embryos. Beta-galactosidase was expressed under the control of the endogenous promoter. (J:58079)

Expression

In Mice Carrying this Mutation:	49 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Hnf1b Mutation: 16 strains or lines available

References

• Original: J:58079 Barbacci E, et al., Variant hepatocyte nuclear factor 1 is required for visceral endoderm specification. Development. 1999 Nov;126(21):4795-805
• All: 10 reference(s)