Nf2^tm1Tyj
Targeted Allele Detail

Summary

• Symbol: Nf2^tm1Tyj
• Name: neurofibromin 2; targeted mutation 1, Tyler Jacks
• MGI ID: MGI:1857941
• Synonyms: Merlin^-, Nf2^-, Nf2^KO2-3
• Gene: Nf2 Location: Chr11:4715845-4799536 bp, - strand Genetic Position: Chr11, 3.0 cM
• Alliance: Nf2^tm1Tyj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:40596
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 3' half of exon 2, all of exon 3 and intervening sequences were replaced by a neomycin cassette inserted in the reverse transcriptional orientation to the gene. RT-PCR analysis demonstrated that an aberrant transcript was expressed from this allele that spliced exon 1 to exon 5. However, immunoblot analysis on extracts derived from homozygous ES cells demonstrated that no stable mutant protein was detectable. (J:40596)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Nf2^tm1Tyj

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nf2 Mutation: 65 strains or lines available

References

• Original: J:40596 McClatchey AI, et al., The Nf2 tumor suppressor gene product is essential for extraembryonic development immediately prior to gastrulation. Genes Dev. 1997 May 15;11(10):1253-65
• All: 9 reference(s)