E2f1^tm1Njd
Targeted Allele Detail

Summary

• Symbol: E2f1^tm1Njd
• Name: E2F transcription factor 1; targeted mutation 1, Nicholas J Dyson
• MGI ID: MGI:1857944
• Synonyms: E2f1-, E2F-1-
• Gene: E2f1 Location: Chr2:154401327-154411812 bp, - strand Genetic Position: Chr2, 76.79 cM
• Alliance: E2f1^tm1Njd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33099
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: All of exon 2 and part of exon 3, encoding residues critical for cyclin and DNA binding, was replaced with a neomycin cassette in antisense orientation. Immunoprecipitation and Western blot analysis demonstrated that no protein was made in fibroblasts isolated from E12.5 homozygous embryos. (J:33099)

Expression

In Mice Carrying this Mutation:	14 assay results
In Structures Affected by this Mutation:	16 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying E2f1^tm1Njd

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any E2f1 Mutation: 28 strains or lines available

References

• Original: J:33099 Yamasaki L, et al., Tumor induction and tissue atrophy in mice lacking E2F-1. Cell. 1996 May 17;85(4):537-48
• All: 30 reference(s)