Apc^tm1Rak
Targeted Allele Detail

Summary

• Symbol: Apc^tm1Rak
• Name: APC, WNT signaling pathway regulator; targeted mutation 1, Raju Kucherlapati
• MGI ID: MGI:1857951
• Synonyms: APC1638, Apc1638N, APC^1638N
• Gene: Apc Location: Chr18:34353977-34455605 bp, + strand Genetic Position: Chr18, 18.53 cM
• Alliance: Apc^tm1Rak page

Microadenoma in Mbd4^tm1Wed/Mbd4^tm1Wed Apc^tm1Rak/Apc⁺ gastrointestinal tract

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:20443
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance cassette was inserted into exon 15 at a site corresponding to codon 1638. This mutation is predicted to result in a truncated protein. RT-PCR analysis detected a mutated transcript produced from this allele, but a truncated protein was not consistently detected by Western blot analysis. (J:20443)

Disease models

Expression

In Mice Carrying this Mutation:	6 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Apc^tm1Rak

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Apc Mutation: 158 strains or lines available

References

• Original: J:20443 Fodde R, et al., A targeted chain-termination mutation in the mouse Apc gene results in multiple intestinal tumors. Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):8969-73
• All: 107 reference(s)