Apex1^tm1Djc
Targeted Allele Detail

Summary

• Symbol: Apex1^tm1Djc
• Name: apurinic/apyrimidinic endonuclease 1; targeted mutation 1, David J Chen
• MGI ID: MGI:1857955
• Synonyms: Ape/Ref^-
• Gene: Apex1 Location: Chr14:51162425-51164596 bp, + strand Genetic Position: Chr14, 26.3 cM, cytoband C3
• Alliance: Apex1^tm1Djc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50702
• Parent Cell Line: JM-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Exon 2, intron 2, exon 3 and part of intron 3 were replaced by a neomycin resistance cassette. (J:50702)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Apex1 Mutation: 16 strains or lines available

Notes

Homozygous mutant embryos proceed to embryonic day 6.5 - 7 with normal morphology and can be genotyped by PCR from day 9.5 embryo stages before their lethality. Pre-implantation blastocysts are gamma ionizing radiation sensitive (J:50702).

References

• Original: J:50702 Ludwig DL, et al., A murine AP-endonuclease gene-targeted deficiency with post-implantation embryonic progression and ionizing radiation sensitivity. Mutat Res. 1998 Oct 21;409(1):17-29
• All: 5 reference(s)