Apc^tm1Tno
Targeted Allele Detail

Summary

• Symbol: Apc^tm1Tno
• Name: APC, WNT signaling pathway regulator; targeted mutation 1, Tetsuo Noda
• MGI ID: MGI:1857966
• Synonyms: 580S, Apc^580s, Apc^{580S flox}, Apc^f, Apc^fl, Apc^loxP, Apc^lx
• Gene: Apc Location: Chr18:34353977-34455605 bp, + strand Genetic Position: Chr18, 18.53 cM
• Alliance: Apc^tm1Tno page

Anterior head defects during embryonic development in Apc^Min/Apc^tm1Tno embryos and rescue of head defects by Ctnnb1^tm4.1Wbm/Ctnnb1⁺

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:43301
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: One loxP site was inserted into intron 13 and the other, with a PGK-neo cassette, into intron 14. Northern blot analysis revealed that RNA expression in the intestinal tract of homozygous mutants was about 30% of that in wild-type mice. Protein expression in the colon of homozygotes by immunohistochemistry was indistinguishable from that of wild-type mice. Western blot analysis of lysates from mouse embryo fibroblasts showed reduced expression of the protein. (J:43301, J:156864)

Disease models

Expression

In Mice Carrying this Mutation:	100 assay results
In Structures Affected by this Mutation:	12 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Apc^tm1Tno

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 6 strains available Cell Lines: 0 lines available
• Carrying any Apc Mutation: 158 strains or lines available

References

• Original: J:43301 Shibata H, et al., Rapid colorectal adenoma formation initiated by conditional targeting of the Apc gene. Science. 1997 Oct 3;278(5335):120-3
• All: 196 reference(s)