Thbd^tm1Emc
Targeted Allele Detail

Summary

• Symbol: Thbd^tm1Emc
• Name: thrombomodulin; targeted mutation 1, Edward M Conway
• MGI ID: MGI:1857982
• Synonyms: TM^cyt
• Gene: Thbd Location: Chr2:148246391-148250108 bp, - strand Genetic Position: Chr2, 73.45 cM
• Alliance: Thbd^tm1Emc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54997
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: The sequences encoding the wild-type protein were replaced with sequences that encoded a truncated form lacking the most carboxy-terminal 34 amino acid residues of the cytoplasmic domain, while retaining an in-frame stop codon. Immunohistochemistry and radioimmunoassay experiments confirmed that the mutant protein was expressed in a similar temporal and spatial manner to the normal protein. (J:54997)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thbd Mutation: 25 strains or lines available

References

• Original: J:54997 Conway EM, et al., Structure-function analyses of thrombomodulin by gene-targeting in mice: the cytoplasmic domain is not required for normal fetal development. Blood. 1999 May 15;93(10):3442-50
• All: 1 reference(s)