Met^tm2Cpo
Targeted Allele Detail

Summary

• Symbol: Met^tm2Cpo
• Name: met proto-oncogene; targeted mutation 2, Carola Ponzetto
• MGI ID: MGI:1858021
• Synonyms: Met^Grb2
• Gene: Met Location: Chr6:17463799-17573979 bp, + strand Genetic Position: Chr6, 7.83 cM, cytoband A2
• Alliance: Met^tm2Cpo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36244
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A mutant human cDNA coding for the transmembrane and cytoplasmic domain of the receptor was inserted in-frame into mouse genomic sequences. The cDNA was followed by a neomycin resistance cassette. A fusion protein consisting of the murine extracellular domain fused to the human transmembrane and cytoplasmic domain carrying the mutation N1358H is produced from this allele. (J:36244)

Expression

In Mice Carrying this Mutation:

3 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Met Mutation: 83 strains or lines available

References

• Original: J:36244 Maina F, et al., Uncoupling of Grb2 from the Met receptor in vivo reveals complex roles in muscle development. Cell. 1996 Nov 1;87(3):531-42
• All: 4 reference(s)