Ift88^tm1Rpw
Targeted Allele Detail

Summary

• Symbol: Ift88^tm1Rpw
• Name: intraflagellar transport 88; targeted mutation 1, Richard P Woychik
• MGI ID: MGI:1859153
• Synonyms: Ift88^{delta2-3betagal}, Ift99^null, Tg737^{delta2-3betaGal}
• Gene: Ift88 Location: Chr14:57661519-57755393 bp, + strand Genetic Position: Chr14, 30.1 cM
• Alliance: Ift88^tm1Rpw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62025
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment containing exons 2 and part of 3 was replaced with a lacZ gene with a splice acceptor site and neomycin selection cassette. RT-PCR analysis demonstrated that the mutation eliminated expression of the wild-type transcript, and whole-mount embryo staining studies revealed that beta-galactosidase is expressed from this allele. (J:62025)

Disease models

Expression

In Mice Carrying this Mutation:	68 assay results
In Structures Affected by this Mutation:	16 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ift88 Mutation: 48 strains or lines available

Notes

Homozygous mutant mice display early to mid-gestation lethality (~embryonic day 9.5), random patterning of the left-right body axis, neural tube defects, pericardial sac expansion, enlarged limb buds, and absent embryonic node cilia.

References

• Original: J:62025 Murcia NS, et al., The Oak Ridge Polycystic Kidney (orpk) disease gene is required for left-right axis determination. Development. 2000 Jun;127(11):2347-55
• All: 21 reference(s)