Uncx^tm1Akis
Targeted Allele Detail

Summary

• Symbol: Uncx^tm1Akis
• Name: UNC homeobox; targeted mutation 1, Andreas Kispert
• MGI ID: MGI:1859373
• Synonyms: Uncx4.1^del
• Gene: Uncx Location: Chr5:139529620-139533934 bp, + strand Genetic Position: Chr5, 78.75 cM
• Alliance: Uncx^tm1Akis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62022
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A deletion of the entire transcription unit of the Uncx4.1 gene was generated by introducing a loxP site 2-kb upstream of the transcription start site and a loxP-flanked neomycin cassette 5-kb to the second exon. Following homologous recombination in ES cells, cre recombinase was transiently expressed in order to excise loxP flanked sequences. ES cell lines carrying the deleted allele were used to generate founder mice. (J:62022)

Expression

In Mice Carrying this Mutation:	22 assay results
In Structures Affected by this Mutation:	14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Uncx Mutation: 28 strains or lines available

References

• Original: J:62022 Leitges M, et al., The paired homeobox gene Uncx4.1 specifies pedicles, transverse processes and proximal ribs of the vertebral column. Development. 2000 Jun;127(11):2259-67
• All: 3 reference(s)