Vil1^tm1Syr
Targeted Allele Detail

Summary

• Symbol: Vil1^tm1Syr
• Name: villin 1; targeted mutation 1, Sylvie Robine
• MGI ID: MGI:1859841
• Synonyms: villin-null
• Gene: Vil1 Location: Chr1:74448543-74474719 bp, + strand Genetic Position: Chr1, 38.54 cM, cytoband C5
• Alliance: Vil1^tm1Syr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57346
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: The open reading frame was disrupted by the insertion of a neomycin selection cassette into exon 2. Normal protein was shown to be absent and no aberrant transcripts were detected via Northern blot and RT-PCR analysis of homozygous mutant mice. Western blot analysis confirmed the absence of encoded protein extracts from intestinal and renal tissue of homozygous mutant mice. (J:57346)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Vil1 Mutation: 52 strains or lines available

References

• Original: J:57346 Ferrary E, et al., In vivo, villin is required for Ca(2+)-dependent F-actin disruption in intestinal brush borders. J Cell Biol. 1999 Aug 23;146(4):819-30
• All: 10 reference(s)