Tnfrsf4^tm1Nik
Targeted Allele Detail

Summary

• Symbol: Tnfrsf4^tm1Nik
• Name: tumor necrosis factor receptor superfamily, member 4; targeted mutation 1, Nigel Killeen
• MGI ID: MGI:1859871
• Synonyms: CD134-, OX40-, OX40 KO
• Gene: Tnfrsf4 Location: Chr4:156098300-156101069 bp, + strand Genetic Position: Chr4, 87.68 cM
• Alliance: Tnfrsf4^tm1Nik page

Lung inflammatory infiltrates, goblet cell hyperplasia, and mucus production are reduced in Tnfrsf4^tm1Nik/Tnfrsf4^tm1Nik mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58979
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin cassette was used to replace four exons, which code for the first 143 amino acids of the protein. Activated T cells from homozygous mutant animals lacked expression of Tnfrsf4 as detected by flow cytometry with the OX86 mAb as well as a soluable ligand fusion protein. (J:58979)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Tnfrsf4^tm1Nik/Tnfrsf4^tm1Nik	involves: 129S4/SvJae * C57BL/6

Phenotypes:

Affected Systems	hm1
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cellular	√
decreased T cell proliferation	√
hematopoietic system	√
decreased T cell proliferation	√
abnormal CD4-positive, alpha-beta T cell physiology	√
abnormal T-helper 2 physiology	√
immune system	√
immune system phenotype	N
decreased T cell proliferation	√
abnormal CD4-positive, alpha-beta T cell physiology	√
abnormal T-helper 2 physiology	√
decreased susceptibility to type I hypersensitivity reaction	√

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnfrsf4 Mutation: 36 strains or lines available

References

• Original: J:58979 Pippig SD, et al., Robust B cell immunity but impaired T cell proliferation in the absence of CD134 (OX40). J Immunol. 1999 Dec 15;163(12):6520-9
• All: 46 reference(s)