Tnfrsf8^tm1Mak
Targeted Allele Detail

Summary

• Symbol: Tnfrsf8^tm1Mak
• Name: tumor necrosis factor receptor superfamily, member 8; targeted mutation 1, Tak Mak
• MGI ID: MGI:1860056
• Synonyms: CD30 -
• Gene: Tnfrsf8 Location: Chr4:144993707-145041734 bp, - strand Genetic Position: Chr4, 78.17 cM
• Alliance: Tnfrsf8^tm1Mak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62854
• Parent Cell Line: E14K (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A construct containing a neomycin resistance gene in the antisense direction was used to replace the second last (or second to last) exon. A dish binding assay indicated the absence of functional Tnfrsf8 protein on the surface of spleen cells isolated from homozygous mutant mice. (J:62854)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tnfrsf8 Mutation: 43 strains or lines available

References

• Original: J:62854 Amakawa R, et al., Impaired negative selection of T cells in Hodgkin's disease antigen CD30-deficient mice. Cell. 1996 Feb 23;84(4):551-62
• All: 28 reference(s)