Thrb^tm1Olc
Targeted Allele Detail

Summary

• Symbol: Thrb^tm1Olc
• Name: thyroid hormone receptor beta; targeted mutation 1, Olivier Chassande
• MGI ID: MGI:1860407
• Synonyms: TRbeta-, TRBetaKO
• Gene: Thrb Location: Chr14:4431611-4809435 bp, - strand Genetic Position: Chr14, 7.08 cM, cytoband A3
• Alliance: Thrb^tm1Olc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:52829
• Parent Cell Line: ENS (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cassette containing both a lacZ coding sequence and a neomycin resistance gene was used to replace exons 4 and 5 which code for the DNA binding domain. This insertion inactivates both the beta1 and beta2 isoforms. RT-PCR was used to demonstrate a lack of mRNA in homozygous mutant animals. (J:52829)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Thrb Mutation: 41 strains or lines available

References

• Original: J:52829 Gauthier K, et al., Different functions for the thyroid hormone receptors TRalpha and TRbeta in the control of thyroid hormone production and post-natal development. EMBO J. 1999 Feb 1;18(3):623-31
• All: 34 reference(s)