Thra^tm1Jas
Targeted Allele Detail

Summary

• Symbol: Thra^tm1Jas
• Name: thyroid hormone receptor alpha; targeted mutation 1, Jacques Samarut
• MGI ID: MGI:1860442
• Synonyms: T3Ralpha-, TRalpha^-
• Gene: Thra Location: Chr11:98631539-98659832 bp, + strand Genetic Position: Chr11, 62.58 cM, cytoband D-E
• Alliance: Thra^tm1Jas page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:41827
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Both alpha1 and alpha2 isoforms encoded by this locus were disrupted by the insertion of a cassette containing both a lacZ coding sequence and a neomyocin resistance gene into exon 2. This construct did not affect the internal promoter within intron 7, allowing expression, as well as increased expression, of delta1 and delta2 isoforms in various organs. A Northern blot confirmed the abrogation of expression of both alpha1 and alpha2 in the small intestines, brain, heart, liver, kidney, and lung. (J:41827)

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thra Mutation: 40 strains or lines available

References

• Original: J:41827 Fraichard A, et al., The T3R alpha gene encoding a thyroid hormone receptor is essential for post-natal development and thyroid hormone production. EMBO J. 1997 Jul 16;16(14):4412-20
• All: 15 reference(s)