Th^tm1Srt
Targeted Allele Detail

Summary

• Symbol: Th^tm1Srt
• Name: tyrosine hydroxylase; targeted mutation 1, Suzanne Roffler-Tarlov
• MGI ID: MGI:1860480
• Synonyms: Th^-, TH-null
• Gene: Th Location: Chr7:142446516-142453732 bp, - strand Genetic Position: Chr7, 88.06 cM
• Alliance: Th^tm1Srt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54692
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette was used to replace sequence from exons 6, 7, and 8. The deleted region encompassed 268 nucleotides encoding a portion of the catalytic domain. Low levels of a peptide, slightly smaller than normal, were detected by Western blot analysis of extracts obtained from homozygous mutant fetuses. The mutant peptide could not be detected by immunohistochemical analysis of fetal adrenals or in fetal or postnatal brains. (J:54692)
• Inheritance: Recessive

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Th Mutation: 58 strains or lines available

References

• Original: J:54692 Rios M, et al., Catecholamine synthesis is mediated by tyrosinase in the absence of tyrosine hydroxylase. J Neurosci. 1999 May 1;19(9):3519-26
• All: 6 reference(s)