Rr128^tm1Masu
Targeted Allele Detail

Summary

• Symbol: Rr128^tm1Masu
• Name: regulatory region 128; targeted mutation 1, M Azim Surani
• MGI ID: MGI:1860836
• Synonyms: H19^SilK, H19^tm1Masu
• Gene: Rr128 Location: unknown Genetic Position: Chr7, Syntenic
• Alliance: Rr128^tm1Masu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63213
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified regulatory region, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP-flanked neomycin resistance cassette replaced the 1.2kb region of the promoter, corresponding to a cis-acting silencer element. This cassette was then excised in ES cells by cre-mediated recombination, resulting in the deletion of the silencer element. (J:63213)
• Inheritance: Dominant

Expression

In Mice Carrying this Mutation:	25 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr128 Mutation: 0 strains or lines available

Notes

This mutation results in a loss of silencing of the paternal allele in many endodermal and other tissues.

References

• Original: J:63213 Drewell RA, et al., Deletion of a silencer element disrupts H19 imprinting independently of a DNA methylation epigenetic switch. Development. 2000 Aug;127(16):3419-28
• All: 2 reference(s)