Med1^tm1Jkr
Targeted Allele Detail

Summary

• Symbol: Med1^tm1Jkr
• Name: mediator complex subunit 1; targeted mutation 1, Janardan K Reddy
• MGI ID: MGI:1861123
• Synonyms: PBP^-
• Gene: Med1 Location: Chr11:98042980-98084119 bp, - strand Genetic Position: Chr11, 61.75 cM, cytoband D
• Alliance: Med1^tm1Jkr page

Histological and ultrastructural alterations in Med1^tm1Jkr/Med1^tm1Jkr placenta

Show the 5 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62272
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exons 12-14 were disrupted with a PGK-neo cassette via homologous recombination resulting in the loss of 2 LXXLL motifs. Northern and Western blot analysis of fibroblasts from homozygous mutant embryos showed absence of gene transcript and protein product. (J:62272)
• Inheritance: Recessive

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Med1 Mutation: 83 strains or lines available

References

• Original: J:62272 Zhu Y, et al., Deletion of PBP/PPARBP, the gene for nuclear receptor coactivator peroxisome proliferator-activated receptor-binding protein, results in embryonic lethality. J Biol Chem. 2000 May 19;275(20):14779-82
• All: 2 reference(s)