Med1^tm1Rgr
Targeted Allele Detail

Summary

• Symbol: Med1^tm1Rgr
• Name: mediator complex subunit 1; targeted mutation 1, Robert G Roeder
• MGI ID: MGI:1861125
• Synonyms: Trap220^-
• Gene: Med1 Location: Chr11:98042980-98084119 bp, - strand Genetic Position: Chr11, 61.75 cM, cytoband D
• Alliance: Med1^tm1Rgr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:61840
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A lacZ-PGK-neo cassette was inserted via homologous recombination resulting in deletion of most of the gene product, including loss of all LXXLL motifs and nuclear receptor coactivator function. Reporter gene assays showed expression was greatly reduced in mouse embyonic fibroblasts cultured from homozygous mutant embyros. (J:61840)
• Inheritance: Recessive

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Med1^tm1Rgr/Med1^tm1Rgr	either: (involves: 129P2/OlaHsd * C57BL/6J) or (involves: 129P2/OlaHsd * 129X1/SvJ)
ht2	Med1^tm1Rgr/Med1^+	either: 129X1/SvJ or (involves: 129X1/SvJ * C57BL/6J)

Phenotypes:

Affected Systems	hm1	ht2
show or hide all annotated terms
cardiovascular system	√
abnormal cardiovascular system morphology	√
abnormal blood vessel morphology	√
abnormal vena cava morphology	√
abnormal myocardial trabeculae morphology	√
thin myocardium compact layer	√
enlarged heart	√
heart hypoplasia	√
trabecula carnea hypoplasia	√
thin ventricular wall	√
abnormal cardiovascular system physiology	√
cellular	√
abnormal cell cycle	√
abnormal neuron apoptosis	√
decreased fibroblast proliferation	√
embryo	√
decreased embryo size	√
abnormal embryonic neuroepithelium morphology	√
endocrine/exocrine glands		√
decreased activity of thyroid gland		√
growth/size/body	√	√
enlarged heart	√
decreased embryo size	√
microcephaly	√
decreased body size		√
decreased body height		√
postnatal growth retardation	√	√
homeostasis/metabolism		√
decreased circulating thyroxine level		√
decreased circulating triiodothyronine level		√
decreased thyroid-stimulating hormone level		√
abnormal metabolism		√
liver/biliary system	√
abnormal liver development	√
mortality/aging	√
embryonic lethality between somite formation and embryo turning, incomplete penetrance	√
embryonic lethality, complete penetrance	√
muscle	√
abnormal myocardial trabeculae morphology	√
trabecula carnea hypoplasia	√
thin myocardium compact layer	√
nervous system	√
abnormal neuron apoptosis	√
abnormal nervous system morphology	√
abnormal embryonic neuroepithelium morphology	√
abnormal brain morphology	√
abnormal forebrain development	√
abnormal telencephalon development	√
respiratory system	√
abnormal bronchus morphology	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

14 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Med1 Mutation: 83 strains or lines available

References

• Original: J:61840 Ito M, et al., Involvement of the TRAP220 component of the TRAP/SMCC coactivator complex in embryonic development and thyroid hormone action. Mol Cell. 2000 Apr;5(4):683-93
• All: 7 reference(s)