Cfb^tm1Hrc
Targeted Allele Detail

Summary

• Symbol: Cfb^tm1Hrc
• Name: complement factor B; targeted mutation 1, Harvey R Colten
• MGI ID: MGI:1861129
• Synonyms: Bf-, Cfb^-, Factor B^-, fB-
• Gene: Cfb Location: Chr17:35075350-35081492 bp, - strand Genetic Position: Chr17, 18.41 cM
• Alliance: Cfb^tm1Hrc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:42679
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129S/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced exons 3 through 7 of the gene. Northern blots of liver from homozygous mutant mice showed no detectable full-length transcript, but did show a smaller, truncated transcript when probes encompassing segments 5' or 3' to the deleted segment were used. Small amounts of truncated, but not intact, protein were detected in peritoneal macrophages from homozygous mutant mice. However, sera from homozygous mutant mice showed no detectable protein activity. (J:42679)

Expression

In Mice Carrying this Mutation:	2 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cfb Mutation: 29 strains or lines available

Notes

ES cell line = D3 (129S2/SvPas) or J1 (129S4/SvJae).

References

• Original: J:42679 Matsumoto M, et al., Abrogation of the alternative complement pathway by targeted deletion of murine factor B. Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8720-5
• All: 76 reference(s)