Zeb1^Tw
Spontaneous Allele Detail

Summary

• Symbol: Zeb1^Tw
• Name: zinc finger E-box binding homeobox 1; twirler
• MGI ID: MGI:1861230
• Gene: Zeb1 Location: Chr18:5591860-5775467 bp, + strand Genetic Position: Chr18, 4.42 cM
• Alliance: Zeb1^Tw page

Inner ear morphology, hearing thresholds, and otoconia of Zeb1^Tw/Zeb1⁺ and Zeb1^Tw/Zeb1^Tw mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Strain of Origin: STOCK PCS

Mutation description

Allele Type: Spontaneous Mutation: Single point mutation Mutation details: A single nucleotide G-to-A substitution at chr18:5592148 (GRCm38) is located 181 bp downstream of Zeb1 exon 1 and 12 bp downstream of exon 1 of overlapping lncRNA Gm10125 on the opposite strand. This mutation does not affect the adjacent splicing site but does disrupt a predicted Myb binding site. An electrophoretic mobility shift assay demonstrated that a probe carrying this mutation does not interfere with Myb binding, unlike a probe with the wild-type sequence. RT-PCR analysis showed that expression of transcripts containing exons 1a and 2 and exons 2 and 3 were increased compared to wild-type controls. (J:177274) Inheritance: Semidominant

Linkage mapping and identification of the Zeb1^Tw mutation

Expression

In Structures Affected by this Mutation:

36 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Zeb1 Mutation: 65 strains or lines available

References

• Original: J:274 LYON MF, Twirler: a mutant affecting the inner ear of the house mouse. J Embryol Exp Morphol. 1958 Mar;6(1):105-16
• All: 9 reference(s)