Igf1^tm2Ts
Targeted Allele Detail

Summary

• Symbol: Igf1^tm2Ts
• Name: insulin-like growth factor 1; targeted mutation 2, Timothy Stewart
• MGI ID: MGI:1861704
• Synonyms: IGF-I^m, Midi allele
• Gene: Igf1 Location: Chr10:87694127-87772904 bp, + strand Genetic Position: Chr10, 43.7 cM
• Alliance: Igf1^tm2Ts page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:37969
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: Hypomorph. In this allele, a site specific insertion of the entire targeting vector occurred instead of homologous recombination. The construct interrupted exon 3 with a neomycin cassette, with a thymidine kinase gene and vector sequences inserted 5' of the endogenous exon 3. RT-PCR analysis demonstrated that a reduced amount of normal transcript was produced from this allele as well as a mutant transcript, and a low, but detectable level of the protein was present in sera of homozygous mice. (J:37969)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igf1 Mutation: 29 strains or lines available

References

• Original: J:37969 Lembo G, et al., Elevated blood pressure and enhanced myocardial contractility in mice with severe IGF-1 deficiency. J Clin Invest. 1996 Dec 1;98(11):2648-55
• All: 5 reference(s)