Blmh^tm1Geh
Targeted Allele Detail

Summary

• Symbol: Blmh^tm1Geh
• Name: bleomycin hydrolase; targeted mutation 1, Gregg E Homanics
• MGI ID: MGI:1861798
• Synonyms: Bh-, Blmh^tm2Geh
• Gene: Blmh Location: Chr11:76836482-76878215 bp, + strand Genetic Position: Chr11, 46.16 cM
• Alliance: Blmh^tm1Geh page

Gross appearance and histopathology of tail dermatitis on Blmh^tm1Geh/Blmh^tm1Geh mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54585
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment containing exon 3 and flanking DNA was replaced with a neomycin selection cassette. The deleted sequences include the region encoding the catalytic cysteine of the protein. Northern blot analysis on total embryonic RNA derived from homozygous mice demonstrated that no transcript was produced from this allele. Western blot and enzymatic activity assays confirmed that no functional protein was made in homozygous mice. (J:54585)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Blmh Mutation: 22 strains or lines available

References

• Original: J:54585 Schwartz DR, et al., The neutral cysteine protease bleomycin hydrolase is essential for epidermal integrity and bleomycin resistance. Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4680-5
• All: 4 reference(s)