Cd80^tm1Shr
Targeted Allele Detail

Summary

• Symbol: Cd80^tm1Shr
• Name: CD80 antigen; targeted mutation 1, Arlene H Sharpe
• MGI ID: MGI:1861804
• Synonyms: B7-, B7-1-, B7.1, CD80-
• Gene: Cd80 Location: Chr16:38277793-38316682 bp, + strand Genetic Position: Chr16, 26.86 cM, cytoband B5
• Alliance: Cd80^tm1Shr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74662
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced the exon encoding the IgV-like domain of the protein. RT-PCR analysis on RNA derived from spleen, thymus and LPS-stimulated B cells of homozygous mice demonstrated that no detectable transcript was produced from this allele. FACS analysis on B cells derived from homozygous mice confirmed that no functional protein was encoded by this allele. (J:74662)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 12 strains available Cell Lines: 0 lines available
• Carrying any Cd80 Mutation: 44 strains or lines available

References

• Original: J:74662 Freeman GJ, et al., Uncovering of functional alternative CTLA-4 counter-receptor in B7-deficient mice. Science. 1993 Nov 5;262(5135):907-9
• All: 152 reference(s)