Cstb^tm1Rm
Targeted Allele Detail

Summary

• Symbol: Cstb^tm1Rm
• Name: cystatin B; targeted mutation 1, Richard M Myers
• MGI ID: MGI:1861915
• Synonyms: Cystatin b^-
• Gene: Cstb Location: Chr10:78261504-78263456 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Cstb^tm1Rm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50587
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 1. Northern blot and RT-PCR analysis on RNA derived from liver of homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on extracts of brain tissue derived from homozygous mice confirmed that no detectable encoded protein was made. (J:50587)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cstb Mutation: 12 strains or lines available

References

• Original: J:50587 Pennacchio LA, et al., Progressive ataxia, myoclonic epilepsy and cerebellar apoptosis in cystatin B-deficient mice. Nat Genet. 1998 Nov;20(3):251-8
• All: 23 reference(s)