Epas1^tm1Rus
Targeted Allele Detail

Summary

• Symbol: Epas1^tm1Rus
• Name: endothelial PAS domain protein 1; targeted mutation 1, David W Russell
• MGI ID: MGI:1861921
• Synonyms: Epas1^-, HIF2alpha^LacZ
• Gene: Epas1 Location: Chr17:87061292-87140838 bp, + strand Genetic Position: Chr17, 56.9 cM
• Alliance: Epas1^tm1Rus page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50964
• Parent Cell Line: JH1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A lacZ gene was inserted and fused in-frame into exon 2, and was followed by a neomycin selection cassette. Western blot analysis indicated that the expression of wild-type transcript was eliminated from this allele. However, a beta-galactosidase fusion protein was expressed under the control of the endogenous promoter. (J:50964)

Expression

In Mice Carrying this Mutation:	30 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Epas1 Mutation: 66 strains or lines available

References

• Original: J:50964 Tian H, et al., The hypoxia-responsive transcription factor EPAS1 is essential for catecholamine homeostasis and protection against heart failure during embryonic development. Genes Dev. 1998 Nov 1;12(21):3320-4
• All: 19 reference(s)