Fas^tm1Osa
Targeted Allele Detail

Summary

• Symbol: Fas^tm1Osa
• Name: Fas cell surface death receptor; targeted mutation 1, Osaka University Medical School
• MGI ID: MGI:1861923
• Synonyms: Fas-, Tnfrsf6^tm1Osa
• Gene: Fas Location: Chr19:34268066-34305172 bp, + strand Genetic Position: Chr19, 29.48 cM
• Alliance: Fas^tm1Osa page

Histopathology of Fas-deficient mice.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64296
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A BetaGal-neo cassette was used to delete a portion of exon 9, which codes for the cytoplasmic domain essential for Fas-mediated apoptosis. Northern blot analysis revealed the expression of a truncated mRNA, representing transcripts from exons 1 though 8 in the thymus, liver, and heart. Immunoprecipitation analysis showed an absence of the wild-type protein as well as a faint presence of what the authors speculated to be a non-functional Tnfrsf6-BetaGal fusion protein. (J:64296)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 7 strains available Cell Lines: 0 lines available
• Carrying any Fas Mutation: 82 strains or lines available

References

• Original: J:64296 Adachi M, et al., Targeted mutation in the Fas gene causes hyperplasia in peripheral lymphoid organs and liver. Nat Genet. 1995 Nov;11(3):294-300
• All: 25 reference(s)