Fcgr3^tm1Jsv
Targeted Allele Detail

Summary

• Symbol: Fcgr3^tm1Jsv
• Name: Fc receptor, IgG, low affinity III; targeted mutation 1, J Sjef Verbeek
• MGI ID: MGI:1861924
• Synonyms: FcgammaRIII^-, Fcgr3^tm1Sjv
• Gene: Fcgr3 Location: Chr1:170878743-170892504 bp, - strand Genetic Position: Chr1, 78.8 cM
• Alliance: Fcgr3^tm1Jsv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:35057
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A hygromycin selection cassette replaced a genomic fragment containing sequences encoding the ligand binding and part of the transmembrane domains. RT-PCR analysis on NK fractions of splenocytes derived from homozygous mice demonstrated that no detectable transcript was produced from this allele. Flow cytometry analysis on NK cells, B lymphocytes, macrophages and neutrophils confirmed that no detectable cell surface protein was encoded by this allele. (J:35057)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Fcgr3^tm1Jsv/Fcgr3^tm1Jsv	B6.129P2-Fcgr3^tm1Jsv/J
hm2	Fcgr3^tm1Jsv/Fcgr3^tm1Jsv	involves: 129P2/OlaHsd * C57BL/6
cx3	Fcgr3^tm1Jsv/Fcgr3^tm1Jsv Sle21^129P2/Ola/Sle21^129P2/Ola	B6.129P2-Fcgr3^tm1Jsv
cx4	Fcer1a^tm1Knt/Fcer1a^tm1Knt Fcer2a^tm1Max/Fcer2a^tm1Max Fcgr1^tm1Jsv/Fcgr1^tm1Jsv Fcgr2b^tm1Ttk/Fcgr2b^tm1Ttk Fcgr3^tm1Jsv/Fcgr3^tm1Jsv	B6.Cg-Fcgr2b^tm1Ttk Fcgr3^tm1Jsv Fcer1a^tm1Knt Fcgr1^tm1Jsv Fcer2a^tm1Max
cx5	Fcgr1^tm1Jsv/Fcgr1^tm1Jsv Fcgr2b^tm1Ttk/Fcgr2b^tm1Ttk Fcgr3^tm1Jsv/Fcgr3^tm1Jsv	involves: 129P2/OlaHsd * 129S4/SvJae * BALB/c * C57BL/6

Phenotypes:

Affected Systems	hm1	hm2	cx3	cx4	cx5
show or hide all annotated terms
cellular		√			√
decreased mast cell degranulation		√
impaired macrophage phagocytosis		√			√
growth/size/body			√
enlarged spleen			√
increased spleen weight			√
hematopoietic system	√	√	√	√	√
enlarged spleen			√
increased spleen weight			√
decreased susceptibility to autoimmune hemolytic anemia		√
abnormal leukocyte cell number	√
increased neutrophil cell number	√
increased B cell number			√
increased activated T cell number			√
abnormal mast cell physiology				√
decreased mast cell degranulation		√
abnormal neutrophil physiology	√
impaired neutrophil recruitment	√
impaired natural killer cell mediated cytotoxicity		√
abnormal macrophage physiology				√
impaired macrophage phagocytosis		√			√
immune system	√	√	√	√	√
enlarged spleen			√
increased spleen weight			√
decreased susceptibility to autoimmune hemolytic anemia		√
abnormal leukocyte cell number	√
increased neutrophil cell number	√
increased B cell number			√
increased activated T cell number			√
abnormal mast cell physiology				√
decreased mast cell degranulation		√
abnormal neutrophil physiology	√
impaired neutrophil recruitment	√
impaired natural killer cell mediated cytotoxicity		√
abnormal macrophage physiology				√
impaired macrophage phagocytosis		√			√
abnormal dendritic cell antigen presentation		√
abnormal type I hypersensitivity reaction		√
increased susceptibility to type I hypersensitivity reaction				√
decreased susceptibility to type II hypersensitivity reaction		√
decreased susceptibility to experimental autoimmune encephalomyelitis	√
decreased susceptibility to induced arthritis	√
increased anti-chromatin antibody level			√
increased anti-double stranded DNA antibody level			√
increased anti-histone antibody level			√
increased anti-single stranded DNA antibody level			√
mortality/aging			N
mortality/aging			N
skeleton	√
decreased susceptibility to induced arthritis	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 5 strains available Cell Lines: 0 lines available
• Carrying any Fcgr3 Mutation: 29 strains or lines available

References

• Original: J:35057 Hazenbos WL, et al., Impaired IgG-dependent anaphylaxis and Arthus reaction in Fc gamma RIII (CD16) deficient mice. Immunity. 1996 Aug;5(2):181-8
• All: 124 reference(s)