Gabrd^tm1Geh
Targeted Allele Detail

Summary

• Symbol: Gabrd^tm1Geh
• Name: gamma-aminobutyric acid (GABA) A receptor, subunit delta; targeted mutation 1, Gregg E Homanics
• MGI ID: MGI:1861926
• Synonyms: delta-, delta⁰
• Gene: Gabrd Location: Chr4:155469437-155482528 bp, - strand Genetic Position: Chr4, 86.17 cM
• Alliance: Gabrd^tm1Geh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64288
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Insertion of a MC1-neomycin resistance cassette into exon 4 disrupted the gene. Northern blot analysis of cerebellar total RNA from homozygous mutant adult mice detected moderate levels of a novel 2.3kb transcript but not the 1.9kb wild-type transcript. Western blot analysis of cerebellar membranes did not detect the wild-type protein in homozygous mutant mice. (J:64288)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gabrd Mutation: 31 strains or lines available

References

• Original: J:64288 Mihalek RM, et al., Attenuated sensitivity to neuroactive steroids in gamma-aminobutyrate type A receptor delta subunit knockout mice. Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12905-10
• All: 61 reference(s)