Gabrg2^tm1Geh
Targeted Allele Detail

Summary

• Symbol: Gabrg2^tm1Geh
• Name: gamma-aminobutyric acid type A receptor, subunit gamma 2; targeted mutation 1, Gregg E Homanics
• MGI ID: MGI:1861927
• Synonyms: Gabrgamma2L^tm1Geh
• Gene: Gabrg2 Location: Chr11:41801030-41891684 bp, - strand Genetic Position: Chr11, 24.8 cM
• Alliance: Gabrg2^tm1Geh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53823
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a genomic fragment containing exon 9. RT-PCR experiments demonstrated that this mutation resulted in the elimination of the expression of the long splice variant but not the short splice variant. Radioligand binding assays confirmed that no functional long variant was expressed from this allele. (J:53823)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gabrg2 Mutation: 41 strains or lines available

References

• Original: J:53823 Homanics GE, et al., Normal electrophysiological and behavioral responses to ethanol in mice lacking the long splice variant of the gamma2 subunit of the gamma-aminobutyrate type A receptor. Neuropharmacology. 1999 Feb;38(2):253-65
• All: 2 reference(s)