Gck^tm1Efr
Targeted Allele Detail

Summary

• Symbol: Gck^tm1Efr
• Name: glucokinase; targeted mutation 1, Shimon Efrat
• MGI ID: MGI:1861929
• Synonyms: GK-
• Gene: Gck Location: Chr11:5850820-5900081 bp, - strand Genetic Position: Chr11, 3.88 cM
• Alliance: Gck^tm1Efr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:28756
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A fragment of the gene spanning exon 2 was replaced by a neomycin resistance cassette, resulting in a deletion and a frame shift of the transcript. Mice heterozygous for the targeted mutation had a 37% reduction in glucose phosphorylation activity in islet homogenates and a 28% reduction of activity in liver compared with wild-type littermates. (J:28756)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gck Mutation: 63 strains or lines available

References

• Original: J:28756 Bali D, et al., Animal model for maturity-onset diabetes of the young generated by disruption of the mouse glucokinase gene. J Biol Chem. 1995 Sep 15;270(37):21464-7
• All: 2 reference(s)