Gt(ROSA)26Sor^tm1Sho
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1Sho
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Stuart Orkin
• MGI ID: MGI:1861931
• Synonyms: gtROSA, R26-beta-gal^fl-STOP, R26^fslz, R26R, R26Rosa-lox-Stop-lox-LacZ, R26RstoplacZ, Rosa26^fsLz, ROSA26-loxP-stoploxP-beta-geo, ROSA26R-LacZ reporter, ROSA26-stop-lacZ
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm1Sho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64291
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert floxed (flanked by loxP) phosphoglycerate kinase-1-cytosine deaminase/phosphoglycerate kinase-1-puromycin (PGK-CD/PGK-Puro) and stop sequences between the splicing acceptor (SA) and the beta-gal-neomycin phosphotransferase fusion gene (beta-geo) sequences of the proviral ROSA 26 locus. Correctly targeted, this resulted in ES cells that were Puro resistant but G418 susceptible, and did not express beta-gal. (J:64291)
• Inheritance: Not Specified

Disease models

Expression

In Mice Carrying this Mutation:

1 assay results

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^tm1Sho

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 4 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

Notes

Mice carrying this mutation are considered to be reporter strains, with successful Cre excision indicated by beta-gal expression in Cre-expressing tissues.

References

• Original: J:64291 Mao X, et al., Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice. Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5037-42
• All: 106 reference(s)