Gt(ROSA)26Sor^tm1Sor
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1Sor
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Philippe Soriano
• MGI ID: MGI:1861932
• Synonyms: Gt(ROSA)26Sor, Gt(ROSA)26Sor^tm1(LacZ)Cos, Gtrosa26^tm1Sor, Gtrosa26^tm/Sor, i-LacZ, loxP-Stop-loxP lacZ reporter, LSL-LacZ, R26-Cond-lacZ, R26-floxstop-lacZ, R26^fslz, R26^LacZ, R26-LNL-LacZ, R26R, R26R-lacZ, R26R^LacZ, R26R^Z, Rosa26 cre reporter, ROSA26^fl, ROSA26-floxed Neo-LacZ, ROSA26-flox-STOP-flox-lacZ, ROSA26^FSLacZ, Rosa26^LacZ, ROSA26-LacZ, Rosa26-lacZ cre reporter, Rosa26-LacZ^loxP, ROSA26::LNL::LacZ, ROSA26-loxP, Rosa26loxP-STOP-lacZ, Rosa26-LoxSTOPLox-LacZ, Rosa26r^-, ROSA26R, ROSA26R^flox, Rosa26R-lacZ, Rosa26RlacZ, Rosa26R^LacZ, ROSA26Sor^tm1Sor, Rosa26^stop-LacZ, ROSA26Xneo^rXlacZ, Rosa2R, Rosa^LoxP, Rosa-LoxP-Stop-LoxP-LacZ, RS, STOP-lacZ
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm1Sor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64292
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector was designed from the original gene trap strain, ROSA beta-geo26, to include a splice acceptor sequence (SA), a neo expression cassette flanked by loxP sites, a lacZ gene, and a polyadenylation (bpA) sequence inserted at a unique Xba1 site approximately 300-bp 5' of the original gene-trap integration site. In addition, a triple polyadenylation sequence was added to the 3' end of the neo expression cassette to prevent transcriptional read-through. Presence of the floxed neo cassette prevents lacZ expression. When crossed with a cre transgenic strain, lacZ is expressed in all cells/tissue where Cre is expressed. (J:64292)
• Inheritance: Not Specified

Disease models

Expression

In Mice Carrying this Mutation:

649 assay results 5 RNA-Seq or microarray experiment(s)

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^tm1Sor

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 7 strains available Cell Lines: 1 line available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:64292 Soriano P, Generalized lacZ expression with the ROSA26 Cre reporter strain [letter]. Nat Genet. 1999 Jan;21(1):70-1
• All: 2800 reference(s)