Hprt1^tm1Detl
Targeted Allele Detail

Summary

• Symbol: Hprt1^tm1Detl
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Peter J Detloff
• MGI ID: MGI:1861939
• Synonyms: hprt^(CAG)146, JO1
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^tm1Detl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:44728
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: A construct was created that introduced a long CAG repeat (n=146 units) into an Xho1 site in exon 3 of a genomic fragment of the locus. An additional 4.9kb genomic fragment, which included exon 4 of the gene, was added to this repeat-containing construct. Appropriately targeted clones had sequences containing the 146-unit CAG repeat, encoding a stretch of 146 glutamines, in place of wild-type exon 3. (J:44728)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

References

• Original: J:44728 Ordway JM, et al., Ectopically expressed CAG repeats cause intranuclear inclusions and a progressive late onset neurological phenotype in the mouse. Cell. 1997 Dec 12;91(6):753-63
• All: 4 reference(s)