Kif3a^tm1Gsn
Targeted Allele Detail

Summary

• Symbol: Kif3a^tm1Gsn
• Name: kinesin family member 3A; targeted mutation 1, Lawrence SB Goldstein
• MGI ID: MGI:1861960
• Synonyms: KIF3A-, Kif3aⁿ, Kif3a^tm1Maz
• Gene: Kif3a Location: Chr11:53458206-53492794 bp, + strand Genetic Position: Chr11, 31.97 cM, cytoband A5-B1
• Alliance: Kif3a^tm1Gsn page

Kif3a^tm1Gsn/Kif3a^tm1Gsn embryos lack cilia on cells in the node

Show the 5 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54575
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 2 was deleted by cre recombinase removal of a targeting vector containing the exon flanked by loxP sites. The resulting transcript was translated out of reading frame, generating a 62 amino acid nonsense protein. Northern and Western blots of heterozygous mutants showed that no fragments or aberrant products were made from the mutant gene. Western blots of homozygous mutants showed an absence of the targeted protein. This allele was generated from Kif3a^tm2Gsn. (J:54575)

Disease models

Expression

In Mice Carrying this Mutation:	24 assay results
In Structures Affected by this Mutation:	19 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kif3a Mutation: 33 strains or lines available

References

• Original: J:54575 Marszalek JR, et al., Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the KIF3A subunit of kinesin-II. Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5043-8
• All: 29 reference(s)