Ptprcap^tm1Tho
Targeted Allele Detail

Summary

• Symbol: Ptprcap^tm1Tho
• Name: protein tyrosine phosphatase receptor type C polypeptide-associated protein; targeted mutation 1, Matt L Thomas
• MGI ID: MGI:1888379
• Synonyms: CD45AP (-)
• Gene: Ptprcap Location: Chr19:4204645-4206709 bp, + strand Genetic Position: Chr19, 3.86 cM, cytoband B
• Alliance: Ptprcap^tm1Tho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:58711
• Parent Cell Line: LSW1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region (exon 2), with the exception of the initiator methionine, was replaced with a neomycin selection cassette. The cassette was inserted in the same transcriptional orientation as the gene in order to preserve the expression of an adjacent loci encoding coronin. Immunoblot analysis showed an absence of encoded protein in lysates derived from the thymi and spleens of homozygous mutant mice. Additional analysis showed that coronin protein levels were not affected by the targeting event. (J:58711)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ptprcap Mutation: 20 strains or lines available

Notes

NOTE: In contrast to the findings reported here, Matsua et al. (J:48387) report that T and B lymphocytes isolated from Ptprcap^tm1Tkda gene-targeted mice fail to respond as well as wild-type to antigen receptor stimulation, and display a diminished association between PTPRC and lymphocyte protein tyrosine kinase (Lck).

References

• Original: J:58711 Kung C, et al., CD45-associated protein is not essential for the regulation of antigen receptor-mediated signal transduction. Eur J Immunol. 1999 Dec;29(12):3951-5
• All: 1 reference(s)