Gsk3b^tm1Jrw
Targeted Allele Detail

Summary

• Symbol: Gsk3b^tm1Jrw
• Name: glycogen synthase kinase 3 beta; targeted mutation 1, James R Woodgett
• MGI ID: MGI:1888887
• Synonyms: gsk3b-, GSK-3beta^-
• Gene: Gsk3b Location: Chr16:37909363-38066446 bp, + strand Genetic Position: Chr16, 26.54 cM, cytoband B4
• Alliance: Gsk3b^tm1Jrw page

Increased bone mass in Gsk3b^tm1Jrw/Gsk3b⁺ mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63274
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced an exon encoding the ATP-binding loop. Southern and Western blots of embryonic fibroblasts (E12.5) showed no detectable DNA and protein of the targeted gene, respectively. (J:63274)

Expression

In Mice Carrying this Mutation:	45 assay results
In Structures Affected by this Mutation:	8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gsk3b Mutation: 113 strains or lines available

References

• Original: J:63274 Hoeflich KP, et al., Requirement for glycogen synthase kinase-3beta in cell survival and NF-kappaB activation [see comments]. Nature. 2000 Jul 6;406(6791):86-90
• All: 35 reference(s)