En1^{tm1(Otx2)Wrst}
Targeted Allele Detail

Summary

• Symbol: En1^{tm1(Otx2)Wrst}
• Name: engrailed 1; targeted mutation 1, Wolfgang Wurst
• MGI ID: MGI:1889804
• Synonyms: En1^Otx2LacZ, En1^OtxLacZ
• Gene: En1 Location: Chr1:120530246-120535719 bp, + strand Genetic Position: Chr1, 52.74 cM, cytoband C2-qter
• Alliance: En1^{tm1(Otx2)Wrst} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:57538
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Knock-in of the Otx2 gene into the endogenous En1 locus. En1 promoter drives expression of a bicistronic Otx2 and IRES-LacZ reporter mRNA. A loxP flanked neomycin resistance casette was removed in vivo by Cre-mediated recombination. (J:57538)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any En1 Mutation: 34 strains or lines available

References

• Original: J:57538 Broccoli V, et al., The caudal limit of Otx2 expression positions the isthmic organizer. Nature. 1999 Sep 9;401(6749):164-8
• All: 8 reference(s)