Myog^tm2Whk
Targeted Allele Detail

Summary

• Symbol: Myog^tm2Whk
• Name: myogenin; targeted mutation 2, William H Klein
• MGI ID: MGI:1913094
• Synonyms: y allele
• Gene: Myog Location: Chr1:134217742-134220286 bp, + strand Genetic Position: Chr1, 58.18 cM
• Alliance: Myog^tm2Whk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:54065
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: A hypomorphic allele was generated by the insertion of a full length myogenin cDNA followed by an SV40 T polyadenylation site and a loxP-flanked neomycin cassette into the first exon and deletion of basepairs +48 to +511. Transcript was present at approximately one-fourth the level of controls in muscle. The mutant genomic sequence still contains the transcription start site and the splice donor of the first exon. (J:54065)

Expression

In Mice Carrying this Mutation:	7 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Myog Mutation: 14 strains or lines available

References

• Original: J:54065 Vivian JL, et al., A hypomorphic myogenin allele reveals distinct myogenin expression levels required for viability, skeletal muscle development, and sternum formation. Dev Biol. 1999 Apr 1;208(1):44-55
• All: 3 reference(s)