Casp1^tm1Sesh
Targeted Allele Detail

Summary

• Symbol: Casp1^tm1Sesh
• Name: caspase 1; targeted mutation 1, Tara Seshadri
• MGI ID: MGI:1927822
• Synonyms: casp-1^-, Casp1 -, Casp1^-/Casp11^129mt, Caspase-1-, ICE -
• Gene: Casp1 Location: Chr9:5298517-5307281 bp, + strand Genetic Position: Chr9, 2.46 cM
• Alliance: Casp1^tm1Sesh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:22964
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin expression cassette was inserted into exon 6, deleting 31 bp of sequence encoding the region of the active site and rendering the sequence out of frame after the insertion. Northern blot analysis on spleen RNA demonstrated an absence of the normal transcript in homozygous mice, and western blot analysis showed that the protein was not expressed in peritoneal macrophages of homozygous mice. This allele was generated in ES cells that lack protein expression of Casp11 (Casp^129mt). (J:22964)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 5 strains available Cell Lines: 0 lines available
• Carrying any Casp1 Mutation: 42 strains or lines available

Notes

The ES cells used to generate this allele contain the linked Casp4^del truncated allele that fails to produce a functional Casp4. Phenotypes associated with this allele may be affected by the presence of the Casp^del allele. J:193522

References

• Original: J:22964 Li P, et al., Mice deficient in IL-1 beta-converting enzyme are defective in production of mature IL-1 beta and resistant to endotoxic shock. Cell. 1995 Feb 10;80(3):401-11
• All: 172 reference(s)