Tg(MMTVneu)202Mul
Transgene Detail

Summary

• Symbol: Tg(MMTVneu)202Mul
• Name: transgene insertion 202, William Muller
• MGI ID: MGI:1930204
• Synonyms: c-neu, erb-B-2, Her2, HER-2/neu, MMTV-c-Neu, MMTV-ErbB2, MMTV-neu, MMTV.neu, MMTVneu, MMTV-NeuT, neu-N, Tg(MMTV-neu)202Mul, TgN (MMTV-neu)
• Transgene: Tg(MMTVneu)202Mul Location: unknown
• Alliance: Tg(MMTVneu)202Mul page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(MMTVneu)202Mul expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Erbb2 (24337)

  Erbb2

  (MGI:95410)

• Mutation details: This transgene has the wild-type form of the rat gene. The transgene consists of an MMTV promoter, an unactivated rat Erbb2 cDNA sequence, and SV40 polyadenylation and splicing signals. Transgene expression was detected in the mammary gland in 5 out of 6 transgenic lines as determined by RNase protection assay. Low levels of transgene transcript were also detected in the salivary gland, spleen, thymus, and lung. (J:56332)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Tg(MMTVneu)202Mul

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

Notes

Multiple transgenic lines were produced, including 202, 169, 510, 721, and 732.

In mice carrying both this transgene and Tg(Trp53R172H)8512Jmr, mammary tumors are first observed after two rounds of pregnancy and lactation at 112 days; the median latency was 154 days. All mice developed mammary tumors before 200 days of age. The neoplasms are of high grade and growth fraction, indicated by variable nuclear and cytological morphology, anaplasia, aneuploidy, and higher rates of mitosis and apoptosis. Metastatic incidence was not reported.

In virgin mice carrying both this transgene and Tg(MMTVTGFA)29Rjc, the mammary trees show extensive lobuloalveolar development comparable to a normal FVB lactating mouse. The alveoli of the bitransgenic mice have a denser cell lining in the walls. Their mammary glands are characterized by epithelial hyperplasia and dysplasia with stromal inflammation. In virgin mice, mammary tumors first arise at about 100 days; the median latency is approximately 180 days. Tumors are multifocal and involve the entire mammary epithelium. Metastatic incidence was not reported.

References

• Original: J:56332 Guy CT, et al., Expression of the neu protooncogene in the mammary epithelium of transgenic mice induces metastatic disease. Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):10578-82
• All: 324 reference(s)