Spi1^tm1Sing
Targeted Allele Detail

Summary

• Symbol: Spi1^tm1Sing
• Name: Spi-1 proto-oncogene; targeted mutation 1, Harinder Singh
• MGI ID: MGI:1930970
• Synonyms: PU.1^-
• Gene: Spi1 Location: Chr2:90912750-90946104 bp, + strand Genetic Position: Chr2, 50.44 cM, cytoband E3
• Alliance: Spi1^tm1Sing page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:20348
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Replacement of a portion of exon 5, including sequences encoding amino acids 200-272, with a neomycin cassette. (J:20348)
• Inheritance: Recessive

Expression

In Mice Carrying this Mutation:	43 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spi1 Mutation: 28 strains or lines available

Notes

This targeted mutation in the Sfpi1 locus causes death of mutant homozygotes in late gestation. These embryos showed a multilineage defect in generation of progenitors for B and T lymphocytes, for monocytes, and for granulocytes. Megakaryocytes and erythroid progenitor cells were produced in normal numbers, though erythroblast maturation was impaired in some mice. The affected lineages require a function acting at the level of a common progenitor cell (J:20348); perhaps the induction of colony stimulating factor genes may represent at least part of the effect.

References

• Original: J:20348 Scott EW, et al., Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages. Science. 1994 Sep 9;265(5178):1573-7
• All: 28 reference(s)