Agrn^tm2Jrs
Targeted Allele Detail

Summary

• Symbol: Agrn^tm2Jrs
• Name: agrin; targeted mutation 2, Joshua R Sanes
• MGI ID: MGI:1930978
• Synonyms: agrn^deltaY
• Gene: Agrn Location: Chr4:156249747-156281945 bp, - strand Genetic Position: Chr4, 88.55 cM
• Alliance: Agrn^tm2Jrs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:55407
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Deletion of exon 28, encoding the "y" alternative splice exon as follows: A lox-P flanked neomycin cassette replaced exon 28, and was excised in ES cells prior to blastocyst injection via Cre-mediated recombination. The final allele has a single lox-P site in place of the exon. (J:55407)
• Inheritance: Recessive

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Agrn Mutation: 102 strains or lines available

References

• Original: J:55407 Burgess RW, et al., Alternatively spliced isoforms of nerve- and muscle-derived agrin: their roles at the neuromuscular junction. Neuron. 1999 May;23(1):33-44
• All: 1 reference(s)